Macherey-Nagel™ NucleoSpin™ 96 PCR Clean-up, 96-well kit for PCR clean up

The NucleoSpin™ 96 PCR Clean-up kit allows direct clean-up of PCR reaction mixtures. Within the procedure the addition of chaotropic salt (Buffer NT) allows a reversible adsorption of the PCR products to the silica membrane of the NucleoSpin™ 96 PCR Clean-up Binding Plates. High purity of the PCR-products is achieved by complete removal of primers, primer-dimers, salts, nucleotides, and proteins (e.g., polymerases, BSA) in subsequent washing steps using Buffer NT3. Highly pure PCR products are finally eluted with Elution Buffer NE (5 mM Tris/HCl, pH 8.5) or water (pH 8.5), and can be used directly for further applications.

• Silica membrane technology
• Processing: manual/automated, vacuum/centrifugation
• Sample material: < 10 0μL PCR reaction mixture
• Elution volume: 75 to 150 μL
• Binding capacity: 15 μg
• Preparation time: 45 min./plate
• Fragment size: 65 bp to 10 kb