Learn More
Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up
Descrizione
NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.
- Two applications in one kit—One buffer to process both sample types, with only one loading step
- High recovery for fragments down to 50 bp—Average recovery from 60–90%
- Minimized elution volume of 15μL—Highly concentrated DNA
- Binding buffer with pH indicator—Ensures optimal binding conditions
- Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
- Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
- Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
- Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)
Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer
Specifica
Specifica
| Colore | pH Indicator: Yellow = correct conditions Green = pH slightly high (adjustment recommended) Blue = pH too high (adjustment necessary) |
| Formato | Colonna per centrifugazione |
| Isolation Technology | Silica Membrane Technology |
| Efficienza | Typical recovery: 70–95% |
| Volume di eluizione | Elution volume: 15–30μL |
| Da utilizzare con (apparecchiatura) | TAE or TBE agarose gels, PCR |
| Include | Binding Buffer NTI (40mL), Wash Buffer NT3 Concentrate (25mL), Elution Buffer NE (13mL), NucleoSpin Gel and PCR Clean-up Columns (50), 2mL Collection Tubes (50) |
| N. di reazioni | 50 preparazioni |
| Quantità | 1 Kit |
| Purezza | A260/A280: 1.8–1.9 |
| Vedi altri risultati |
Sicurezza e movimentazione
- Attenzione
- Tossicità acuta Categoria 4
- PERICOLO A LUNGO TERMINE PER L'AMBIENTE ACQUATICO Cronico 3
- H302-Nocivo se ingerito.
- H412-Nocivo per gli organismi acquatici con effetti di lunga durata.
- MIXTURE LIST-Contiene: guanidinium thiocyanate
Facendo clic su Invia, l'utente riconosce che potrebbe essere contattato da Fisher Scientific in merito al feedback fornito in questo modulo. Non condivideremo le vostre informazioni per altri scopi. Tutte le informazioni di contatto fornite saranno conservate in conformità con la nostra Politica sulla privacy. Informativa sulla privacy.