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  6. Macherey-Nagel™ NucleoSpin™ Plasmid Transfection-grade, Mini kit for ultrapure plasmid DNA

Macherey-Nagel™ NucleoSpin™ Plasmid Transfection-grade, Mini kit for ultrapure plasmid DNA

Product Code. 15503636
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Product Code. 15503636 Supplier Macherey-Nagel™ Supplier No. 740490.50

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Description

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

  • Purifies high-copy plasmids for transfection
  • Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
  • Rapid purification from E. coli via manual, vacuum or centrifugation processing
  • Ultra-fast procedure using mini spin columns simplifies the work-flow
  • Downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application, such as cloning and sequencing

Specifications

Endotossine batteriche <50 EU/ng DNA
Content And Storage Room temperature (18 - 25 °C)
Formato Mini Spin Column
Isolation Technology Silica Membrane and Endotoxin-reduction Technology
Tipo di campione Bacterial Culture (E. coli, High-copy Plasmids)
Efficienza Binding capacity: 35 μg
Volume di eluizione Elution Volume: 30 - 50 μL
Tipo di prodotto finale Transfection-grade plasmid DNA
Da utilizzare con (apparecchiatura) Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
Da utilizzare con (applicazione) Rapid purification of plasmids for transfection
Include NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A
N. di campioni 50
Purezza A260/A280: 1.80 - 1.85
Volume del campione Up to 5 mL
Size < 15 kbp
Durata del test 14 min/6 preps
Resa 15 - 30 μg
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Safety and Handling

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Signal Word
  • Pericolo
 Hazard Category
  • Liquido infiammabile Categoria 3
  • Tossicità acuta Categoria 4
  • Corrosione/irritazione della pelle Categoria 2
  • Lesione oculare grave/irritazione oculare Categoria 2
  • Sensibilizzante delle vie respiratorie Categoria 1
  • Tossicità specifica per organi bersaglio (esposizione singola) Categoria 3
 Hazard Statement
  • H226-Liquido e vapori infiammabili.
  • H302-Nocivo se ingerito.
  • H315-Provoca irritazione cutanea.
  • H319-Provoca grave irritazione oculare.
  • H334-Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.
  • H336-Può provocare sonnolenza o vertigini.
 Precautionary Statement
  • P210-Tenere lontano da fonti di calore, superfici riscaldate, scintille, fiamme e altre fonti di innesco. Vietato fumare.
  • P260-Non respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
  • P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
  • P261-Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
  • P342+P311-In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI/un medico/ .
 Supplemental information
  • MIXTURE LIST-Contain: sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt/guanidine hydrochloride/trie
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