Macherey-Nagel™ NucleoZOL, one phase RNA purification

NucleoZOL is designed for the isolation of total RNA (small and large RNA) in a single fraction or in separate fractions from a variety of sample materials.

• No chloroform, no phase separation, easy procedure
• High RNA yield and purity from any sample material
• RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
• Small and large RNA in one or in separated fractions
• RNA ready for use in a variety of downstream applications: RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Sample material:

• < 1 x 10⁷ cultured cells, bacteria, and yeast
• < 100 mg human/animal/plant tissue
• < 0.4 mL (viral) fluids

Typical yield:

• Total RNA
  • Liver: 6-8 μg/mg tissue
  • Kidney, spleen: 3-4 μg/mg tissue
  • Muscle, brain, lung: 0.5-1.5 μg/mg
  • Cultured cells: 4-10 μg/10⁶ cells
• Large RNA
  • Liver: 5-7 μg/mg tissue
  • Kidney, spleen: 3-4 μg/mg tissue
  • Muscle, brain, lung: 0.5-1.5 μg/mg
  • Cultured cells: 3-8 μg/10⁶ cells