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Invitrogen™ OncoStat Panel, SNAP-ChIP™ Spike-in

Codice prodotto. 17183239 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
10 reactions
100 reactions
Dimensione della confezione:
10 reazioni
100 reazioni
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Codice prodotto. Quantità unitSize
17183239 100 reactions 100 reazioni
17168855 10 reactions 10 reazioni
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Codice prodotto. 17183239 Fornitore Invitrogen™ N. del fornitore A47355

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Descrizione

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation) uses DNA-barcoded semi-synthetic nucleosomes bearing distinct histone mutations as spike-in ChIP controls.

  • SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation) uses DNA-barcoded semi-synthetic nucleosomes bearing distinct histone mutations as spike-in ChIP controls.
  • Homogenous, fully defined standards faithfully represent target mononucleosomes in the experimental sample
  • Nucleosomes are subjected to rigorous quality control for lot-to-lot consistency
  • Unique DNA barcodes can be distinguished from experimental sample genomes
  • Spike-ins provide a direct readout of antibody performance in ChIP
  • Ability to monitor technical variability between samples
  • Defined standards enable universal normalization across experiments
  • SNAP-ChIP panels are directly compatible with your current ChIP workflow, with semi-synthetic nucleosomes bearing the mutation of interest immunoprecipitated and processed alongside sample chromatin.
  • Users can directly monitor antibody capability (specificity and enrichment) in a ChIP experiment by measuring the recovery of on- and off-target nucleosomes. The SNAP spike-ins can be further used as a defined standard to normalize your ChIP data, thereby controlling for technical variability across experiments.
  • The OncoStat Panel consists of a pool of nucleosomes harboring seven well-studied histone H3.3 mutations that have been implicated in cancer (histone H3.3K4M, H3.3K9M, H3.3K27M, H3.3G34R, H3.3G34V, H3.3G34W and H3.3K36M) plus a wild-type H3.3 control. Further, each of these eight nucleosomes is wrapped by two independent DNA barcodes (16 total barcodes in the panel), allowing for an internal technical replicate in each ChIP reaction.
  • A single spike-in of the panel allows users to check antibody specificity by examining the post-IP recovery of on- versus off-target SNAP-ChIP nucleosomes, supporting the generation of high quality ChIP data.
TRUSTED_SUSTAINABILITY

Specifica

Descrizione Recombinant Nucleosome Panel
Content And Storage Store at -5 to -30°C.
N. di test 100 Reactions
Quantità 100 reactions
Product Line OncoStat, SNAP-ChIP
Product Type SNAP-ChIP Panel

For Research Use Only. Not for use in diagnostic procedures.

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