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OPTIZYME™ ApaI, Fisher BioReagents™
Marca: Fisher Bioreagents BP8025-1
Dettagli aggiuntivi : Peso : 0.01000kg
Descrizione
5'...G G G C C^C...3'
3'...C^C C G G G...5'
Supplied with: 10X OPTIZYME Buffer 1
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 1: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 100%
- Buffer 2: 20 - 50%
- Buffer 3: 0 - 20%
- Buffer 4: 20 - 50%
- Buffer 5: 0 - 20%
- 10mM Tris-HCl (pH 7.4 at 25°C), 50mM NaCl, 1 mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- Incubation at 30°C results in a 2-fold increase in activity.
- ApaI is inhibited by salt concentrations above 50 mM.
- ApaI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain.
Methylation Effects:
Dam: Never overlaps - no effect
Dcm: May overlap - cleavage impaired
- 5'...GGGCCm5CW GG...3'
- 5'...GGGCCm5C G...3'
- 5'...m5C GGGCCm5C G...3'
Specifica
10 U/μL | |
37°C | |
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with ApaI | |
Keep container tightly closed | |
GGGCC.C | |
Liquid |
25 to 50% Water, >50% Glycerin | |
7.4 | |
10mM Tris HCl (pH 7.4 at 25°C), 50mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
5000 U | |
Apal |