OPTIZYME™ BamHI, Fisher BioReagents™

Descrizione

5'...G^G A T C C...3'
3'...C C T A G^G...5'

Conditions for 100% Activity:

• 1X OPTIZYME Buffer BamHI: 10mM Tris-HCl (pH 8.0 at 37°C), 5mM MgCl₂, 100mM KCl, 0.02% Triton X-100 and 0.1mg/ml BSA.
• Incubate at 37°C

Supplied with: 10X OPTIZYME BamHI Buffer
Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 20 - 50%™
• Buffer 2: 100%
• Buffer 3: 20 - 50%
• Buffer 4: 100%™
• Buffer 5: 50 - 100%™

™star activity occurs at greater than 5-fold overdigestion (5 units × 1 hour)
Storage Buffer:
10mM Tris-HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with BamHI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of BamHI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Note: Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity.
Compatible Ends: BclI, BglII, Bsp143I, MboI, PsuI.
Methylation Effects:
Dam: completely overlaps - no effect.
Dcm: may overlap - no effect.
CpG: may overlap - no effect.
EcoKI: never overlaps - no effect.
EcoBI: never overlaps - no effect.