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OPTIZYME™ BstEII (Eco91I), Fisher BioReagents™

Marca:  Fisher Bioreagents BP8038-1

Dettagli aggiuntivi : Peso : 0.01000kg

Codice prodotto. 11924802

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Descrizione

Descrizione

5'...G^G T N A C C...3'
3'...C C A N T G^G...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl)2 , 100mM NaCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 20 - 50%
  • Buffer 2: 20 - 50%
  • Buffer 3: 100%
  • Buffer 4: No reaction
  • Buffer 5: 50 - 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with BstEII.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of BstEII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Compatible Ends: G^GTSACC - MaeIII, NmuCI; G^GTWACC - MaeIII
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: May overlap - no effect
    • 5'...Cm5CW GGTNACm5CW GG...3'
  • 3'...G GWm5CCANTG GWm5CC...5'
  • (No cleavage effect)
  • CpG: May overlap - no effect
    • 5'...m5C GGTNACm5C G...3'
  • 3'... Gm5CCANTG Gm5C...5'
  • (No cleavage effect)
  • EcoKI: May overlap - effect not determined
  • EcoBI: May overlap - effect not determined
  • Specifica

    Specifica

    10 U/μL
    37°C
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BstEII
    Keep container tightly closed
    G.GTNACC
    Liquid
    25 to 50% Water, >50% Glycerin
    7.4
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    2000 U
    BstEII
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