OPTIZYME™ Csp6I (CviQI), Fisher BioReagents™

Name: OPTIZYME&trade; Csp6I (CviQI), Fisher BioReagents&trade;
Brand: Fisher Bioreagents
SKU: 11934822
Price: 425.00 EUR

Marca: Fisher Bioreagents BP8068-1

Dettagli aggiuntivi : Peso : 0.01000kg

Codice prodotto. 11934822

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Descrizione

5'...G^T A C...3'
3'...C A T^G...5'

Supplied with: 10X OPTIZYME Buffer 1
Conditions for 100% Activity:

1X OPTIZYME Buffer 1: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl)₂ and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Buffer 1: 100%
Buffer 2: 50 - 100%
Buffer 3: 0 - 20%
Buffer 4: 50 - 100%
Buffer 5: 0 - 20%

Storage Buffer:

10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with Csp6I.

Compatible Ends: FspBI, NdeI, Tru1I, VspI

Methylation Effects:

Dam: never overlaps - no effect

Dcm: never overlaps - no effect

CpG: may overlap - no effect

5'...m5C GTAm5C G...3'

3'... Gm5CAT Gm5C...5'

(No effect on cleavage)

EcoKI: never overlaps - no effect

EcoBI: never overlaps - no effect

Specifica

Concentrazione	10 U/μL
Temperatura incubatore	37°C
Da utilizzare con (applicazione)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Csp6I
Content And Storage	Keep container tightly closed
Sito di taglio	G.TAC
Forma	Liquid

Componenti	25 to 50% Water, >50% Glycerin
pH	7.4
Tampone di conservazione	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantità	1500 U
Product Type	Csp6 I

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OPTIZYME™ Csp6I (CviQI), Fisher BioReagents™

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