OPTIZYME™ HpaII, Fisher BioReagents™

Name: OPTIZYME&trade; HpaII, Fisher BioReagents&trade;
Brand: Fisher Bioreagents
SKU: 11974792
Price: 540.00 EUR

Marca: Fisher Bioreagents BP8032-1

Dettagli aggiuntivi : Peso : 0.01000kg

Codice prodotto. 11974792

Spedizione stimata: 13-05-2024
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Descrizione

5'...C^C G G...3'
3'...G G C^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with HpaII.

Compatible Ends: Bsp119I, Bsu15I, Hin1I, MaeII, NarI, Psp1406I, SsiI, TaqI, XmiI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: Completely overlaps - blocked

(Cleavage is blocked)

EcoKI: Never overlaps - no effect

EcoBI: Never overlaps - no effect

Specifica

Concentrazione	10 U/μL
Temperatura incubatore	37°C
Tampone di conservazione	10mM Tris HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantità	1000 U
Product Type	HpaII

Componenti	25 to 50% Water, >50% Glycerin
Da utilizzare con (applicazione)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
Content And Storage	Keep container tightly closed
Sito di taglio	C.CGG
Forma	Liquid

Suggerimenti di prodotto SDS

Descrizione