OPTIZYME™ NcoI, Fisher BioReagents™

Name: OPTIZYME&trade; NcoI, Fisher BioReagents&trade;
Brand: Fisher Bioreagents
SKU: 11994772
Price: 293.00 EUR

Marca: Fisher Bioreagents BP8017-1

Dettagli aggiuntivi : Peso : 0.01000kg

Codice prodotto. 11994772

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Descrizione

5'...C^C A T G G...3'
3'...G G T A C^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Buffer 1: 20 - 50%
Buffer 2: 20 - 50%
Buffer 3: 20 - 50%
Buffer 4: 100%
Buffer 5: 50 - 100%

Storage Buffer:

10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with NcoI.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of NcoI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Note: Low salt, high glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.

Compatible Ends: AflIII, PscI, BtgI, Eco130I, FatI, PagI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: Never overlaps - no effect

EcoKI: Never overlaps - no effect

EcoBI: Never overlaps - no effect

Specifica

Concentrazione	10 U/μL
pH	7.4
Tampone di conservazione	10mM Tris HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantità	500 U
Product Type	NcoI

Componenti	25 to 50% Water, >50% Glycerin
Da utilizzare con (applicazione)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Nco I
Content And Storage	Keep container tightly closed
Sito di taglio	C.CATGG
Forma	Liquid

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OPTIZYME™ NcoI, Fisher BioReagents™

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