missing translation for 'onlineSavingsMsg'
Learn More
Learn More
OPTIZYME™ T4 Polynucleotide Kinase, Fisher BioReagents™
Marca: Fisher Bioreagents BP8098-1
Dettagli aggiuntivi : Peso : 0.02000kg
Descrizione
OPTIZYME™ T4 PNK catalyzes the transfer of γ-phosphate from ATP to the 5'-OH group of DNA, RNA, oligonucleotides and nucleoside 3'-monophosphates.
Applications and Features:
- OPTIZYME™ T4 PNK labels 5'-termini of nucleic acids (for use as: probes for hybridization or transcript mapping, primers for PCR or DNA sequencing, and markers for gel-electrophoresis)
- 5'-phosphorylation of oligonucleotides, PCR products, and other DNA or RNA prior to ligation
- Phosphorylation of PCR primers
- Removal of 3'-phosphate groups
- Active in buffers for OPTIZYME restriction enzymes, T4 DNA Ligase and Reverse Transcriptases
Supplied With:
- 10X OPTIZYME T4 PNK Buffer 1 (for forward reaction): 500mM Tris-HCl (pH7.6 at 25°C), 100mM MgCl2, 50mM DTT, 1mM spermidine
- 10X OPTIZYME T4 PNK Buffer 2 (for exchange reaction): 500mM imidazole-HCl (pH6.4 at 25°C), 180mM MgCl2, 50mM DTT, 1mM spermidine, 1mM ADP
- 24% PEG 6000 Sol for T4 PNK: 24% (w/v) polyethylene glycol 6000
Quality Control:
- Endo-, exodeoxyribonuclease and ribonuclease-free
- Functionally tested for labeling of 5'-termini of DNA
Specifica
| 10 U/μL | |
| 7.5 | |
| 10X OPTIZYME™ T4 PNK Buffer 1 (for forward reaction): 500mM Tris HCl (7.6 pH at 25°C), 100mM MgCl2, 50mM DTT, 1mM spermidine, 10X OPTIZYME™ T4 PNK Buffer 2 (for exchange reaction): 500mM imidazole-HCl | |
| E. coli cells with a cloned pse T gene of bacteriophage T4 | |
| T4 Polynucleotide Kinase |
| Colorless | |
| Probes for hybridization or transcript mapping, primers for PCR or DNA sequencing and markers for Gel electrophoresis, removal of 3'-phosphate groups | |
| -20°C | |
| 500 U | |
| Liquid |