Thermo Scientific™ Oxoid™ Biochemical Identification System (O.B.I.S.) Salmonella

The genus Salmonella consists of over 2,400 serotypes that may cause acute infectious gastroenteritis frequently associated with food poisoning. Since many Salmonella species infect domestic animals, either clinically or subclinically, cases of Salmonella food poisoning usually originate from animal sources.

Use O.B.I.S. Salmonella to reduce the need for a full biochemical identification of suspect colonies on common selective Salmonella media.

• Simple—easy to implement
• Rapid—results in 6 minutes of testing suspect colonies
• Non-carcinogenic substrates

Identification of Salmonella spp. relies on primary isolation of the organism on selective enteric media. However, there are several other genera among the Enterobacteriaceae also capable of growth on such media and which can have similar colony morphology to Salmonellae. The lack of PYRase and NPA activity in Salmonella spp. can be used to differentiate them from Citrobacter spp., which possess PYRase activity;^{1, 2, 3}and Proteus, Morganella and Providencia spp., which have NPA activity⁴.

We have developed a new system for PYRase testing⁵ using a non-carcinogenic substrate in response to health concerns associated with the use of β-naphthylamide (a potent carcinogen)⁶. The PYRase area on the O.B.I.S. Salmonella is impregnated with L-pyroglutamic acid 7-amino-4-methylcoumarin (7AMC)⁵. Dimethylaminocinnamaldehyde is used as a color development reagent. The enzymatic hydrolysis of the substrate produces a purple color on addition of the O.B.I.S. PYR Developing Solution⁵.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific™ brand.