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DiscoverX PathHunter™ eXpress P2RY1 U2OS β-Arrestin GPCR Assay

Codice prodotto. 17324059
Encompass
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Quantità:
1,000 dp (10 x 96-well)
Dimensione della confezione:
1 set
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Codice prodotto. Quantità unitSize
17324059 1,000 dp (10 x 96-well) 1 set
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Codice prodotto. 17324059 Fornitore DiscoverX N. del fornitore 930941E3CP5L

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Descrizione

The PathHunter™ eXpress P2RY1 U2OS β-Arrestin GPCR Assay measures P2RY1 (GPCR) activity via recruitment of β-Arrestin2. eXpress kits contain all assay materials: cells, reagents, and plates.

The PathHunter™ P2RY1 β-Arrestin eXpress Kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. ATP) based activation of P2RY1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology (www.discoverx.com/technologies-platforms/enzyme-fragment-complementation-technology). The included cells overexpress PK-tagged P2RY1 and EA-tagged β-Arrestin 2. Activation of P2RY1 stimulates the recruitment of β-Arrestin 2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Specifica

Assay Entry β-Arrestin-2 recruitment
Da utilizzare con (applicazione) Assay-Ready eXpress Kits
Primary Agonist 2-(methylthio)-ATP
Quantità 1,000 dp (10 x 96-well)
Target Entry P2RY1
Linea cellulare U2OS
Bersaglio P2RY1
Titolo del prodotto
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