BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer

Some cyanine-type dyes (PE-Cy5, PerCP-Cy5.5, PE-Cy7, APC-Cy7, APC-H7, and others) and non-cyanine tandem dyes (eg, PE-CF594) are known to bind to human and mouse monocytes, macrophages, and other leukocyte subsets, though the exact mechanism is not clear. BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces this nonspecific background staining, while not impacting specific surface staining or cell viability. This buffer is added directly to leukocytes or added to fluorescent reagent cocktails prior to staining leukocytes. This reagent may be complemented by others including i) BD Horizon™ Brilliant Stain Buffer, which mitigates dye-dye interactions amongst BD Horizon™ Brilliant fluorochromes, and ii) BD Fc Block™ reagents. The latter can reduce potential nonspecific staining caused by fluorescent antibodies that bind to Fc receptors expressed by human and mouse cells. For optimal and reproducible surface staining results, BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer should be used with cyanine-like and other tandem dye-conjugated reagents that can potentially stain cells nonspecifically. Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.