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Applied Biosystems™ PicoPure™ RNA Isolation Kit
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N. di campioni:
40 samples
Dimensione della confezione:
1 set
Descrizione
The ARCTURUS™ PicoPure™ RNA Isolation Kit is designed to recover high-quality total RNA consistently from fewer than ten cells, even from a single cell. This kit is designed to provide consistent, efficient RNA recovery without sacrificing quality. Small volume elution allows you to maximize your recovery of RNA from small numbers of cells for use with your gene expression analysis.
Key Product Features:
Greater sensitivty—isolate RNA from even a single cell
Superior efficiency—maximize RNA recovery from samples
Streamlined concentration—elute with small volumes
Flexible application—compatible with many tube sizes
Integrated contamination prevention—column lids ensure that samples remain RNase free
Isolate RNA from Even a Single Cell
The PicoPure™ RNA Isolation Kit is engineered to recover high-quality total RNA consistently from fewer than ten cells. The high recovery rate attained using this kit is valuable not only for small samples containing picograms of RNA but also for samples containing up to 100μg of RNA. Since the RNA elutes in as little as 10μl of buffer, it is ready for gene expression analysis without any vacuum concentration. This kit is available in two sizes, enough for 40 or 200 isolations.
Get Superior RNA Recovery
Small, valuable samples often cannot be replaced. Performing gene expression studies on these precious samples requires getting maximum RNA recovery. Comparative studies show that, with small, RNA-poor cell or tissue samples, the PicoPure™ Kit dramatically outperforms other commercial total RNA isolation kits in efficiency.
Eliminate Time-consuming RNA Concentration
The simple isolation protocol consists of extracting cellular RNA, then loading the extract onto the specially designed MiraCol™ Purification Column to bind the RNA. After washing away impurities, the RNA elutes in only 10μl of buffer, ready for use without vacuum concentration or transfer into a new reaction tube.
High Yield Purification Columns
The PicoPure™ RNA Isolation Kit features unique, high-recovery MiraCol™ Purification Columns, engineered to maximize recovery from microscopic samples. The columns have convenient integrated lids to help ensure that samples remain RNase free. In addition, the Kit comes complete with all necessary buffers, an easy-to-follow User Guide, optimized protocols for handling Laser Capture Microdissected (LCM) samples, and protocols for isolating RNA from larger samples.
Deliver RNA Ready for Amplification and Microarray Analysis
RNA isolated with the PicoPure™ RNA Kit is ready, without vacuum concentration, for amplification with the RiboAmp™ RNA Amplification Kit. The two kits together allow for highly reproducible gene expression results.
Retain Low-abundance mRNA
The PicoPure™ RNA Isolation Kit preserves mRNA in all abundance classes through the isolation process, minimizing the loss of important genes of interest. Low, medium, and high-abundance genes are consistently recovered and available for expression analysis.
Maintain High RNA Quality
The PicoPure™ RNA isolation process allows for high-quality RNA. Total cellular RNA prepared from LCM samples remains intact after isolation, increasing confidence in subsequent molecular analysis.
Key Product Features:
Isolate RNA from Even a Single Cell
The PicoPure™ RNA Isolation Kit is engineered to recover high-quality total RNA consistently from fewer than ten cells. The high recovery rate attained using this kit is valuable not only for small samples containing picograms of RNA but also for samples containing up to 100μg of RNA. Since the RNA elutes in as little as 10μl of buffer, it is ready for gene expression analysis without any vacuum concentration. This kit is available in two sizes, enough for 40 or 200 isolations.
Get Superior RNA Recovery
Small, valuable samples often cannot be replaced. Performing gene expression studies on these precious samples requires getting maximum RNA recovery. Comparative studies show that, with small, RNA-poor cell or tissue samples, the PicoPure™ Kit dramatically outperforms other commercial total RNA isolation kits in efficiency.
Eliminate Time-consuming RNA Concentration
The simple isolation protocol consists of extracting cellular RNA, then loading the extract onto the specially designed MiraCol™ Purification Column to bind the RNA. After washing away impurities, the RNA elutes in only 10μl of buffer, ready for use without vacuum concentration or transfer into a new reaction tube.
High Yield Purification Columns
The PicoPure™ RNA Isolation Kit features unique, high-recovery MiraCol™ Purification Columns, engineered to maximize recovery from microscopic samples. The columns have convenient integrated lids to help ensure that samples remain RNase free. In addition, the Kit comes complete with all necessary buffers, an easy-to-follow User Guide, optimized protocols for handling Laser Capture Microdissected (LCM) samples, and protocols for isolating RNA from larger samples.
Deliver RNA Ready for Amplification and Microarray Analysis
RNA isolated with the PicoPure™ RNA Kit is ready, without vacuum concentration, for amplification with the RiboAmp™ RNA Amplification Kit. The two kits together allow for highly reproducible gene expression results.
Retain Low-abundance mRNA
The PicoPure™ RNA Isolation Kit preserves mRNA in all abundance classes through the isolation process, minimizing the loss of important genes of interest. Low, medium, and high-abundance genes are consistently recovered and available for expression analysis.
Maintain High RNA Quality
The PicoPure™ RNA isolation process allows for high-quality RNA. Total cellular RNA prepared from LCM samples remains intact after isolation, increasing confidence in subsequent molecular analysis.
Specifica
Specifica
| Content And Storage | Extraction buffer, purification reagents, purification columns, microcentrifuge tubes. Store at room temperature |
| Formato | Spin Column |
| Isolation Technology | Spin Column |
| Tipo di campione | Laser Capture Microdissection (LCM), Tissue (Frozen) |
| Tipo di prodotto finale | Total RNA |
| Compatibilità ad alta produttività | Not High-throughput Compatible (Manual) |
| Da utilizzare con (apparecchiatura) | ArcturusXT™ LCM Instrument |
| Da utilizzare con (applicazione) | Microarray Analysis, Real-Time Quantitative PCR (qPCR) |
| N. di campioni | 40 samples |
| Linea di prodotti | PicoPure |
| Vedi altri risultati |
Sicurezza e movimentazione
Identificativo prodotto
- RNA Isolation Kit
- Pericolo
- Tossicità acuta Categoria 4
- PERICOLO A LUNGO TERMINE PER L'AMBIENTE ACQUATICO Cronico 3
- Lesione oculare grave/irritazione oculare Categoria 2
- Liquido infiammabile Categoria 2
- Liquido infiammabile Categoria 3
- Corrosione/irritazione della pelle Categoria 1C
- Corrosione/irritazione della pelle Categoria 2
- Sensibilizzazione della pelle Categoria 1
- H225-Liquido e vapori facilmente infiammabili.
- H226-Liquido e vapori infiammabili.
- H302-Nocivo se ingerito.
- H314-Provoca gravi ustioni cutanee e gravi lesioni oculari.
- H315-Provoca irritazione cutanea.
- H317-Può provocare una reazione allergica cutanea.
- H319-Provoca grave irritazione oculare.
- H412-Nocivo per gli organismi acquatici con effetti di lunga durata.
- P210-Tenere lontano da fonti di calore, superfici riscaldate, scintille, fiamme e altre fonti di innesco. Vietato fumare.
- P233-Tenere il recipiente ben chiuso.
- P242-Utilizzare solo utensili antiscintillamento.
- P243-Prendere precauzioni contro le scariche elettrostatiche.
- P261-Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P264-Lavare accuratamente dopo l'uso.
- P270-Non mangiare, né bere, né fumare durante l'uso.
- P273-Non disperdere nell'ambiente.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P301+P310-IN CASO DI INGESTIONE: contattare immediatamente un CENTRO ANTIVELENI/un medico/
- P302+P352-IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua/.
- P303+P361+P353-IN CASO DI CONTATTO CON LA PELLE (o con i capelli): togliere immediatamente tutti gli indumenti contaminati. Sciacquare la pelle/fare una doccia.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P330-Sciacquare la bocca.
- P337+P313-Se l'irritazione degli occhi persiste, consultare un medico.
- P370+P378-In caso d'incendio: utilizzare per estinguere.
- P403+P235-Conservare in luogo fresco e ben ventilato.
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
- MIXTURE LIST-Contiene: 2-Mercaptoethanol, Thiocyanic acid, ethanol
For Research Use Only. Not for use in diagnostic procedures.
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