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Thermo Scientific™ Substrati per precipitazione beta-galattosidasi Pierce™
Detect beta-galactosidase expression from vectors, plasmids, or DNA fragments with this chromogenic substrate.
Marca: Thermo Scientific™ 34060
Dettagli aggiuntivi : Peso : 1.81440kg
Descrizione
Thermo Scientific™ Pierce beta-Galactosidase Precipitating Substrates is a chromogenic substrate for beta-galactosidase that is used to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene.
X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate forβ-Galactosidase. It yields a blue precipitate, is soluble in dimethylformamide, and can be used to detect vectors, plasmids or DNA fragments that contain the β-Galactosidase gene.Highlights:
- See item-specific features
X-Gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside)
- Chromogenic substrate for β-Galactosidase
- Yields a blue precipitate
- Soluble in DMF
- Can be used to detect vectors, plasmids, or DNA fragments that contain the β-Galactosidase gene
IPTG (Isopropyl-β-D-thiogalactopyranoside)
- Maximizes the expression of genes in expression vectors
- When screening a lambda bacteriophage library with an antibody, the probability of success can be increased by preventing the expression of the fusion protein until the plaques are well established
- By placing a nitrocellulose filter containing the inducer IPTG onto the plate and continuing growth at 37°C, the expression of the potential β-galactosidase-cDNA fusion protein can be induced after three to four hours of plaque growth; the nitrocellulose filter is then screened with antibodies
- IPTG is also used in combination with X-Gal to detect active β-galactosidase
- Practical in applications using Bacteriophage M13 and Igt11 vectors
Specifica
| Store at or below -20°C. | |
| Powder | |
| 1 g | |
| IPTG (Isopropyl β-D-thiogalactopyranoside) |
For Research Use Only. Not for use in diagnostic procedures.