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Thermo Scientific™ Colonne di microcentrifugazione Pierce™ C-18

Codice prodotto. 10627275 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
25 colonne
50 colonne
96 Tips
96 puntali
96 x 10 μL Tips
96 x 20 μL Tips
96 x 200 μL Tips
Product Type:
Column
Pipette Tip
Spin Tip
Dimensione della confezione:
200µL
20µL
pezzi 25
pezzi 50
pezzi 96
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Codice prodotto. Quantità Product Type unitSize
10627275 96 x 10 μL Tips Pipette Tip pezzi 96
11814141 25 colonne Column pezzi 25
11824141 50 colonne Column pezzi 50
13404219 96 Tips Spin Tip pezzi 96
10615555 96 puntali Pipette Tip pezzi 96
31941184 96 x 20 μL Tips Spin Tip 20µL
31941777 96 x 200 μL Tips Spin Tip 200µL
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Codice prodotto. 10627275 Fornitore Thermo Scientific™ N. del fornitore 87782
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Description

Thermo Scientific Pierce C18 Spin Columns (10–150 μL) and Spin Tips (10–100 μL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods.

C18 Spin Tips for Peptide Desalting and Cleanup

Pierce™ C18 spin tips are designed for rapid, reproducible peptide desalting, cleanup, and concentration prior to mass spectrometry analysis. Utilizing reversed-phase C18 chemistry, these tips help enable efficient peptide binding and recovery while removing salts, detergents, and other contaminants that can interfere with downstream applications.

Used in combination with Pierce Spin Tip Adaptors (Catalog No. A40011776) and low protein binding microcentrifuge tubes (Catalog No. 90410), C18 spin tips support a simple centrifugation-based workflow that delivers consistent and reliable results across a wide range of sample inputs.

Features of C18 Spin Tips

  • Reliable – proven reversed-phase C18 chemistry
  • Efficient – effectively removes interfering contaminants to improve spectral quality
  • Streamlined – simple workflow using tip adaptor and centrifugation steps

Applications

  • Peptide desalting prior to LC-MS analysis
  • Cleanup following protein digestion workflows
  • Removal of salts, buffers, and contaminants
  • Peptide concentration and sample preparation

How to Use

C18 spin tips use a bind–wash–elute workflow performed by centrifugation. Tips are placed into a tip adaptor seated in a microcentrifuge tube for each step. Simply activate and equilibrate prior to adding your acidified sample. To enhance peptide binding repeat the sample loading step one time. Lastly, wash 2-3 times and elute into a fresh tube using an acetonitrile solution. Refer to Table 1 in the Figures for recommended buffers, volumes and spin speeds for each tip size.

TRUSTED_SUSTAINABILITY

Specifications

Volume letto 10 μL
Descrizione Pierce C-18 Tips, 10 μL bed
Da utilizzare con (apparecchiatura) Microcentrifuge, Mass Spectrometer
Product Type Pipette Tip
Product Line Pierce
Content And Storage Store at room temperature.
Starting Material Peptides, Protease-digested Protein
Tipo di prodotto finale Peptides
Metodo di rilevamento Mass Spectrometry
Formato Tip
Workflow Step Sample Clean Up
Quantità 96 x 10 μL Tips
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