missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. 13484209

Thermo Scientific™ Pierce™ ChIP-grade Protein A/G Magnetic Beads

Codice prodotto. 13484209 Sfoglia Tutto Thermo Scientific Prodotti
Cambia vista
Click to view available options
Quantità:
5 mL
Dimensione della confezione:
5mL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Codice prodotto. Quantità unitSize
13484209 5 mL 5mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
To receive the discount customers must purchase 2 of the same product at list price in a single order to receive 30% discount. There is no limit to the multiples of 2 that customers can buy. Use promo code ”27974” to get your promotional price
Codice prodotto. 13484209 Fornitore Thermo Scientific™ N. del fornitore 26162

Please to purchase this item. Need a web account? Register with us today!

663.60 EUR valido fino al 2026-05-15
MIGLIOR PREZZO in promozione! Usa il codice promo "27974" per avere il tuo prezzo promozionale.
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
To receive the discount customers must purchase 2 of the same product at list price in a single order to receive 30% discount. There is no limit to the multiples of 2 that customers can buy. Use promo code ”27974” to get your promotional price

Descrizione

Efficiently isolate chromatin-bound DNA by immunoprecipitation (chromatin IP) for subsequent quantitation by PCR using Thermo Scientific™ Pierce Magnetic ChIP Kit.

Efficiently isolate chromatin-bound DNA by immunoprecipitation (chromatin IP) for subsequent quantitation by PCR using Thermo Scientific™ Pierce Magnetic ChIP Kit.

Additional Information and Online Ordering

The Pierce Magnetic ChIP Kit contains sufficient reagents to perform 30 chromatin immunoprecipitation (ChIP) assays with appropriate controls using an optimized protocol. The kit includes reagents for cell lysis, capture of protein-DNA complexes, reversal of crosslinking, and DNA isolation. The blocked Pierce Protein A/G Magnetic Beads provide high binding capacity, low non-specific background, and work with many antibody species. These beads can be used manually with a magnetic stand or with automated platforms, such as the Thermo Scientific KingFisher Instruments. ChIP-validated and quality-guaranteed antibodies are also available for use with the Pierce Magnetic ChIP Kit.

Highlights:

  • Rapid – obtain purified DNA that is ready for quantitative PCR in about 8 hours

  • Efficient and reproducible – micrococcal nuclease digestion and nuclear lysis are highly optimized

  • Sensitive – obtain results with as few as 10,000 cells (1 x 104)

  • Low background – Pierce Protein A/G Magnetic Beads are blocked in a non-DNA-containing reagent to minimize background

  • Complete – optimized positive control reagents are included: RNA polymerase II antibody and GAPDH promoter PCR primers

Includes:

Kit contains reagents for chromatin preparation, IP, DNA purification, and positive controls (antibody and primers)

Requires:

In vivo crosslinker (such as formaldehyde), micro-tip sonicator (such as Misonix™ Sonicator 3000), ChIP qualified antibody of choice, PCR primers for DNA sequence of interest, PCR master mix (containing a dye such as SYBR™ Green, if qPCR is desired), and a qPCR instrument

Recommended for:

  • Determine sites of specific protein-DNA interactions on genomic DNA by PCR or ChIP-Seq
  • Monitor the effects of histone modifications or chemical agents on DNA binding
TRUSTED_SUSTAINABILITY

Specifica

Descrizione Pierce ChIP-grade Protein A/G Magnetic Beads
Content And Storage Store in original container protected from direct sunlight in a dry, cool and well-ventilated area.
Product Line Pierce
Quantità 5 mL
Bersaglio Antibody
Tipo Magnetic Bead (ChIP grade)
Tipo di legante Protein A/G
Sufficiente per Approx. 250 Assays

For Research Use Only. Not for use in diagnostic procedures.

Titolo del prodotto
Selezionare un problema

Facendo clic su Invia, l'utente riconosce che potrebbe essere contattato da Fisher Scientific in merito al feedback fornito in questo modulo. Non condivideremo le vostre informazioni per altri scopi. Tutte le informazioni di contatto fornite saranno conservate in conformità con la nostra Politica sulla privacy. Informativa sulla privacy.