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Thermo Scientific™ Substrato per western blot ECL Pierce™

Codice prodotto. 10455145 Sfoglia Tutto Thermo Scientific Prodotti
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Codice prodotto. Quantità Dimensione della confezione
10455145 Kit da 250 ml 250mL
10590624 Kit da 50 ml 50mL
10005943 Kit da 500 ml 500mL
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Codice prodotto. 10455145 Fornitore Thermo Scientific™ N. del fornitore 32209
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    279.00€
    245.65€ / 250mL
    Risparmia 33.35€
    11% Sconto
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Beskrivning

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce ECL Plus Western Blotting Substrate characteristics include:

  • Sensitivity: low picogram
  • Stability: 1-hr working solution stability, 1-year kit stability at 4°C
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: 1 to 2 hours
  • Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 μg/mL)
  • Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 μg/mL)

Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.

Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.

TRUSTED_SUSTAINABILITY

Specifikationer

Product Type Substrate
Metodo di rilevamento Chemiluminescence
Forma Liquid
Da utilizzare con (applicazione) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce
Quantità Kit da 250 ml
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:1-15K (0.07-1μg/mL), 1°: 1:1K (0.2- 10μg/mL)
Sensibilità Low Picogram
Signal Duration 1 to 2 hr.
Sufficiente per 2500 sq. cm
Contenuto kit Luminol/Enhancer, 125 mL, Stable Peroxide Buffer, 125 mL
Content And Storage Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
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For Research Use Only. Not for use in diagnostic procedures.

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