Thermo Scientific™ Pierce™ Protein G IgG Plus Orientation Kit, 2 mL

The immobilization method is called orientation because antibodies are first captured to the affinity resin through their Fc regions, which are the target binding sites of Protein A and Protein G ligands. Thus, the antibodies are bound with their antigen-binding sites oriented outward. DSS crosslinker reacts with primary amines (side chain of lysine residues) of the Protein A/G and affinity-bound antibody, thereby covalently and permanently attaching the proteins together.

Highlights:

• Contaminant-free antigen purification – enables antigen purification without contamination (co-elution) of antibody

• Covalent, leak-resistant antibody immobilization – high-capacity binding of IgG to Protein A or Protein G agarose, followed by efficient crosslinking with DSS crosslinker

• High-quality agarose resin – crosslinked 6% beaded agarose with recombinant Protein A or Protein G

• Gentle binding and crosslinking conditions – coupling antibody in standard PBS

• Oriented immobilization – binds and crosslinks IgG with the antigen-binding sites directed outward, able to capture passing antigen in the mobile phase without steric hindrance

• Complete kits – include all the reagents needed to immobilize the antibody

• Better than DMP – less antibody leaching than traditional orientation method with dimethyl pimelimidate crosslinker

Includes:

Protein A or G columns, disuccinimidyl suberate (DSS), PBS pack, blocking buffer, binding/wash buffer, elution buffer, and column accessories