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Description
Thermo Scientific™ Pierce Iminobiotin and Biotin Resins provide for unusual affinity purification experiments involving clean-up or removal of streptavidin or avidin protein components.
Immobilized biotin binds avidin and streptavidin very strongly, making it appropriate for non-reversible binding applications. Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components.
Highlights:
- Enable purification or removal of avidin or streptavidin samples
- Resins are crosslinked beaded agarose onto which biotin or iminobiotin are covalently immobilized
- Use Biotin Agarose for non-reversible binding (e.g., removal of avidin components from a sample)
- Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)
Spezifikation
Spezifikation
| Descrizione | Pierce Iminobiotin Agarose |
| Content And Storage | Upon receipt store at 4°C. |
| Product Line | Pierce |
| Quantità | 5 mL |
| Bersaglio | Streptavidin |
| Tipo | Agarose |
| Fase stazionaria | Biotin Derivative |
| Formato | Bottle |
| Tipo di colonna | Affinity, Agarose Resin |
For Research Use Only. Not for use in diagnostic procedures.
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