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Thermo Scientific™ Soluzioni di substrato NBT/BCIP Pierce™
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Marca: Thermo Scientific™ 34042
242.25 EUR valido fino al 2024-11-29
Usa il codice promo "21683" per avere il tuo prezzo promozionale.
Descrizione
Thermo Scientific™ Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).Highlights:
AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph
Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor
1-Step™ NBT/BCIP
- Ideal for immunoblotting applications
- Minimal assay-to-assay variability
1-Step NBT/BCIP Plus Suppressor
- Ideal for staining tissues assayed with calf intestinal alkaline phosphatase without the interference caused by endogenous phosphatases
- Contains 1mM levamisole to inhibit endogenous phosphatase activity
Specifica
Soluzione di substrato 1-Step NBT/BCIP Pierce | |
Colorimetric | |
Nitrocellulose, PVDF | |
250 mL | |
Alkaline Phosphatase |
Substrate | |
Liquid | |
1-Step™ | |
Alkaline Phosphatase | |
Upon receipt store product at 4°C. Product is shipped at ambient temperature. |
For Research Use Only. Not for use in diagnostic procedures.