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Thermo Scientific™ Soluzioni di substrato NBT/BCIP Pierce™

Codice prodotto. 10434175 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
250 mL
Dimensione della confezione:
250mL
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10434175 250 mL 250mL
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Codice prodotto. 10434175 Fornitore Thermo Scientific™ N. del fornitore 34042

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274.55 EUR valido fino al 2026-06-30
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Beschreibung

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Thermo Scientific™ Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).

Highlights:

  • AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples

  • Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph

  • Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor

1-Step™ NBT/BCIP

  • Ideal for immunoblotting applications
  • Minimal assay-to-assay variability

1-Step NBT/BCIP Plus Suppressor

  • Ideal for staining tissues assayed with calf intestinal alkaline phosphatase without the interference caused by endogenous phosphatases
  • Contains 1mM levamisole to inhibit endogenous phosphatase activity
TRUSTED_SUSTAINABILITY

Spezifikation

Descrizione Soluzione di substrato 1-Step NBT/BCIP Pierce
Product Type Substrate
Metodo di rilevamento Colorimetric
Forma Liquid
Membrane Compatibility Nitrocellulose, PVDF
Product Line 1-Step
Quantità 250 mL
Substrate Alkaline Phosphatase
Reattività Alkaline Phosphatase
Content And Storage Upon receipt store product at 4°C. Product is shipped at ambient temperature.

For Research Use Only. Not for use in diagnostic procedures.

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