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Thermo Scientific™ PEG4-SPDP (PEGylated, long-chain SPDP crosslinker)

Codice prodotto. 11802164 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
100 mg
Dimensione della confezione:
100mg
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Codice prodotto. 11802164

Marca: Thermo Scientific™ 26128

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Descrizione

Long (25.7A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 4-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.

Thermo Scientific™ Pierce PEG4-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a 4-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.

SPDP reagents have an amine-reactive N-hydroxysuccinimide (NHS) ester at one end and a suflhydryl-reactive 2-pyridyldithio group at the opposite end. PEG4-SPDP has a 4-unit polyethylene glycol spacer arm, which confers greater solubility to the crosslinker and linked proteins compared to crosslinkers having only hydrocarbon spacers. Pyridyldithiol reagents produce disulfide-containing linkages that can be cleaved with reducing agents such as dithiothreitol (DTT). Crosslinking experiments with SPDP reagents are not limited to those involving proteins. Any of a variety of molecules with primary amines and sulfhydryl groups can be modified or crosslinked using an SPDP reagent.

Highlights:

  • Efficient amine conjugation – NHS ester provides effective conjugation to lysines, N-termini of peptides and other primary amines

  • Reversible sulfhydryl conjugation – pyridyldithio group enables disulfide linkage with reduced cysteines and other sulfhydryl groups, providing a stable but cleavable bond

  • Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and often enhance the solubility of attached molecules

  • Spacer arm – connects primary amines and sulfhydryl targets at distances up to 25.7 angstroms

Specifications:

  • Chemical name (simplified): 2-pyridyldithiol-tetraoxatetradecane-N-hydroxysuccinimide
  • Chemical formula: C23H33N3O9S2
  • Molecular weight: 559.65
  • Spacer arm: 25.7 angstroms
  • Form: straw to amber colored oil
  • Solubility: dissolve 2mg in 179μL DMSO or DMF to make 20mM stock for each use
TRUSTED_SUSTAINABILITY

Specifica

Chemical Reactivity Amine-Sulfhydryl
Cleavable By Thiols
Descrizione PEG4-SPDP
Peso molecolare 559.65
PEGylated Yes
Lunghezza braccio spaziatore 25.7 Å
Content And Storage Upon receipt store at -20°C protected from moisture.
Permeabilità celle No
Shipping Condition Ambient
Linea di prodotti Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety NHS Ester, Pyridyldisulfide
Spacer Medium (10 to 30 Å)
Forma Liquid
Quantità 100 mg
Solubilità Water
Formato Standard
Solubile in acqua Yes
Product Type Crosslinker
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For Research Use Only. Not for use in diagnostic procedures.

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