Thermo Scientific™ Peroxidase Suppressor

The immunoperoxidase method is a commonly used detection method for immunostaining of cells and tissues. However, the analysis often is complicated by the presence of endogenous peroxidase and “pseudoperoxidase” activity in the cells and tissues. This presence results in nonspecific staining when a substrate such as diaminobenzidine (DAB) is present. Nonspecific staining is difficult to distinguish from the immunochemical marker, making it difficult to distinguish the final location of the antigen of interest. The typical method of inhibiting endogenous peroxidase activity involves hydrogen peroxide or hydrogen peroxide in methanol. The peroxidase suppressor inhibits peroxidase activity even more effectively than the method using hydrogen peroxide in methanol. Plus, the Peroxidase Suppressor does not affect antibody activity. Peroxidase Suppressor also can be used on blots of whole cell supernatant prior to the addition of the horseradish peroxidase (HRP) marker.

Highlights:

Stable

One component

Ready-to-use

Effectively inhibits peroxidase activity without interfering with antigen-antibody interactions