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Thermo Scientific™ Pierce™ Peroxide Assay Reagent B

Codice prodotto. 11871355 Sfoglia Tutto Thermo Scientific Prodotti
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50 mL
Dimensione della confezione:
50mL
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11871355 50 mL 50mL
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Codice prodotto. 11871355 Fornitore Thermo Scientific™ N. del fornitore 23282
  • ONLINE EXCLUSIVE PRICE
    179.00€
    141.41€ / 50mL
    Risparmia 37.59€
    21% Sconto
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Spedizione stimata: 26-11-2026
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Descrizione

Detect and measure hydrogen peroxide levels (H2O2) in biological samples using an iron (Fe) and xylenol orange (XO) reagent for microplates or tubes.

Thermo Scientific Pierce Peroxide Assay Reagent B is a formulation consisting of 125μM xylenol orange in water with sorbitol. This formulation is one of the component of Quantitative Peroxide Assay Kit used for detecting and measuring hydrogen peroxide levels based on the oxidation of ferrous to ferric ion in the presence of xylenol orange, present in the biological and other liquid samples.

Features of Peroxide Assay Reagent B:

  • Convenient—no proprietary reagents involved and remove the variability, difficulty and added expense of formulating the reagents yourself
  • Aqueous-compatible formulations— the formulation includes sorbitol, which provides enhanced sensitivity with non-lipid samples

    Applications of Pierce Quantitative Peroxide Assay Kits:

  • General hydrogen peroxide (H2O2) measurement in protein samples
  • Quantitation of peroxides in detergents
  • Assessment of the effects of peroxides on cellular activity
  • Determination of protein glycation; Measurement of peroxide accumulation in lipids

    In these assay kits, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.

    Related Products

    Pierce™ Quantitative Peroxide Assay Kit (Aqueous) (Cat. No. 23280)

    Pierce™ Quantitative Peroxide Assay Kit (Lipid) (Cat. No. 23285)

    Pierce™ Peroxide Assay Reagent A (Cat. No. 23281)

    Pierce™ Peroxide Assay Reagent C (Cat. No. 23283)

    • TRUSTED_SUSTAINABILITY

    Specifica

    Content And Storage Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
    Descrizione Pierce Peroxide Assay Reagent B
    Saggio Peroxide Assay Reagent B
    Metodo di rilevamento Absorbance
    Linea di prodotti Pierce
    Product Type Quantitative Peroxide Assay
    Specificità Not Target-Specific
    Da utilizzare con (applicazione) Solution-based Detection, Absorbance
    Da utilizzare con (apparecchiatura) Spectrophotometer, Microplate Reader
    Quantità 50 mL

    For Research Use Only. Not for use in diagnostic procedures.

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