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Description
Thermo Scientific Pierce Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.
Features of Premium Grade EDC:
- EDC—carbodiimide crosslinker for conjugation between carboxylates and amines
- High quality—identity and purity confirmed by several tests
- Product integrity—enhanced level of testing and characterization compared to standard grade
- Lot retention—ample supply of past lots retained to ensure future process testing
- Change management—Change Control Notification (CCN) service
- Consistent manufacture—batch-specific manufacturing documentation review
Compared to the standard grade product, Premium Grade EDC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. EDC is 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride, a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation. In the presence of N-hydroxysulfosuccinimide (sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding sulfo-NHS.
Specifications of Premium Grade EDC
We manufacture crosslinking reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
- Visual: White crystalline powder, free of foreign matter
- Identity: IR scan shows only peaks characteristic of EDC
- Water content: Karl Fischer titration, ≤ 2%
- Trace metals: All metals, including lead, ≤ 50 ppm
Applications:
- Conjugate carboxyl and amino groups among peptides and proteins
- Couple haptens to immunogenic carrier proteins (e.g., attach a peptide to KLH)
- Immobilize peptide antigens to affinity-purify antibodies (Cat. No. 20266)
- Create NHS-activated, amine-reactive labeling compounds
- Crosslink proteins to carboxyl coated beads or surfaces
- Activate nanoparticles with amine-reactive sulfo-NHS esters
Product References:
- Crosslinker Application Guide -- search for recent literature references for this product
Related Products
- EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (Cat. No. 22980)
- Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide) (Cat. No. PG82071)
Specifications
Specifications
| Chemical Reactivity | Amine-Carboxyl |
| Cleavable | No |
| Peso molecolare | 191.7 |
| PEGylated | No |
| Lunghezza braccio spaziatore | 0.0 Å |
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Permeabilità celle | No |
| Shipping Condition | Room Temperature |
| Linea di prodotti | Pierce |
| Labeling Method | Chemical Labeling |
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Safety and Handling
- Pierce Premium Grade, EDC
- Attenzione
- Lesione oculare grave/irritazione oculare Categoria 2
- Corrosione/irritazione della pelle Categoria 2
- Tossicità specifica per organi bersaglio (esposizione singola) Categoria 3
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
- H335-Può irritare le vie respiratorie.
- P260-Non respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P405-Conservare sotto chiave.
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
- MIXTURE LIST-Contain: N'-(ethylcarbonimidoyl)-N,N-dimethylpropane-1,3-diamine monohydrochloride
For Research Use Only. Not for use in diagnostic procedures.
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