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Thermo Scientific™ Resina alla proteina A/G UltraLink™ Pierce™
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Thermo Scientific™ Pierce Protein A/G UltraLink Resin is an extremely durable beaded polyacrylamide support to which recombinant Protein A/G has been immobilized for use in IgG purification methods.
UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.
Highlights:
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Immobilized Protein A/G – specifically binds IgG from several mammalian species, especially most subtypes of human and rabbit IgG
Standard capacity – loading of immobilized Protein A/G provides a binding capacity of approx. 20mg human IgG per milliliter of resin
UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications
Properties of Thermo Scientific UltraLink Resin:
- Support pH Stability: 1 to 13
- Average Particle Size: 50 to 80 microns
- Exclusion Limit: greater than 2,000,000 daltons
- Average Surface Area: greater than 250m2 per gram of beads
- Average Pore Volume: greater than 1.2mL per gram of beads (> 60% of bead volume)
- Pore Size: 500 to 1000 angstroms (50 to 100nm)
- Maximum Linear Velocity: 3000cm per hour
- Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)
Spezifikation
Spezifikation
| Product Type | Resin |
| Product Line | Pierce, UltraLink |
| Tipo di colonna | UltraLink Affinity Resin |
| Formato | Bottle |
| Stationary Phase | Protein A/G |
| Purification Target | IgG Antibodies |
| Content And Storage | Upon receipt store resin at 4°C. Product is shipped at ambient temperature. |
| Quantità | 10 mL |
For Research Use Only. Not for use in diagnostic procedures.
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