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Thermo Scientific™ Pierce™ Quantitative Peroxide Assay Kit (Lipid)
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Detect and measure hydrogen peroxide levels in biological samples using an iron and xylenol orange reagent with Thermo Scientific™ Pierce Quantitative Peroxide Assay Kits.
The Thermo Scientific Pierce Quantitative Peroxide Assay Kit (lipid-compatible formulation) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange (XO) reagent.
This lipid-compatible peroxide assay kit detects peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. The kit's formulation may be used with lipid-containing samples without first extracting the lipids; however, because this kit does not contain sorbitol, the assay is less sensitive. Most proteins do not interfere with this assay, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.
Features of the Quantitative Peroxide Assay Kit:
• Peroxides—detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples
• Convenient—no proprietary reagents involved but the ready-to-use kit removes the variability, difficulty and added expense of formulating the reagents yourself
Applications:
• General hydrogen peroxide (H2O2) measurement in protein samples
• Quantitation of peroxides in detergents
• Assessment of the effects of peroxides on cellular activity
• Determination of protein glycation; Measurement of peroxide accumulation in lipids
In this assay, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With this lipid-compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560 nm. Peroxide levels in test samples can be determined by calculation from the known extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.
Related Products
Pierce™ Quantitative Peroxide Assay Kit, Aqueous (Cat. No. 23280)
Pierce™ Peroxide Assay Reagent A (Cat. No. 23281)
Pierce™ Peroxide Assay Reagent B (Cat. No. 23282)
Pierce™ Peroxide Assay Reagent C (Cat. No. 23283)
Specifica
Specifica
| Content And Storage | Sufficient For: 100 tube assays or 500 microplate assays with lipid-containing samples • Reagent A, 1 mL • Reagent C, 4 x 25 mL Store at 4°C. |
| Descrizione | Pierce Quantitative Peroxide Assay Kit (Lipid) |
| Saggio | Quantitative Peroxide Assay Kit |
| Metodo di rilevamento | Colorimetric |
| Linea di prodotti | Pierce |
| Product Type | Quantitative Peroxide Assay |
| Specificità | Not Target-Specific |
| Sufficiente per | 100 Tube Assays or 500 Microplate Assays |
| Da utilizzare con (applicazione) | Solution-based Detection, Absorbance |
| Da utilizzare con (apparecchiatura) | Spectrophotometer, Microplate Reader |
| Vedi altri risultati |
For Research Use Only. Not for use in diagnostic procedures.
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