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Thermo Scientific™ Pierce™ Renilla-Firefly Luciferase Dual Assay Kit
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Simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.
The Thermo Scientific Pierce Renilla-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.
Features of the Renilla-Firefly Luciferase Dual Assay Kit:
- Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
- Sensitive—measure Green Renilla and red firefly luciferase activities in the same sample
- Fast—no quenching step required, unlike in traditional sequential dual assays
- Multiplex—capable of quantitating two cellular activities in the same sample(s)
The Pierce Renilla-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Green Renilla and Red Firefly luciferase activity. Green Renilla luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. As shown in the animation video below, this reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.
Includes:
Cell lysis buffer, reaction buffer, and substrates coelenterazine and D-luciferin
Requires:
Green Renilla and Red Firefly luciferase reporters; Filter sets at 525nm±20nm for Green Renilla Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as the Varioskan ALF or the Varioskan LUX microplate readers; Injector required for assessing more than 24 wells at a time.
Applications:
- Study two regulatory elements at the same time
- Monitor two signaling pathways simultaneously
- Enable studying more than one target per screen (e.g. off-target effects)
Green Renilla produces brighter bioluminescent signal than firefly and native Renilla luciferases. The bioluminescent signal (λmax= 535nm) produced by Green Renilla luciferase protein results from the oxidation of coelenterazine. The second assay uses red firefly luciferase, which is a mutant form of the Japanese firefly luciferase Luciola cruciata. This luciferase produces a red-shifted emission spectrum (λmax= 613nm) that results from the oxidation of D-luciferin in the presence of ATP.
Related Products
Pierce™ Renilla-Firefly Luciferase Dual Assay Kit (Cat. No. 16185)
Specifica
Specifica
| Tipo | Assay Kit |
| Bersaglio | Luciferase, Firefly Luciferase, Green Renilla Luciferase |
| Sufficiente per | 100 Dual Luciferase Assays |
| Quantità | 100 Reaction Kit |
| Content And Storage | Sufficient For: 100 dual-luciferase assays in microplate wells • Renilla-Firefly Dual Assay Buffer, 5 mL (store at 4°C) • 100X Coelenterazine, 50 μL (store at -80°C) • D-Luciferin, 3 mg (store at 4°C) • Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature) Upon receipt, store kit at -80°C, or store individual components as indicated above. |
| Saggio | Reporter Enzyme, Luciferase Reporter Assay |
| Compatible Cells | Mammalian Cells |
| Metodo di rilevamento | Bioluminescence |
| Da utilizzare con (apparecchiatura) | Luminometer |
| Formato | 96-well Plate, 384-well Plate |
| Vedi altri risultati |
Safety and Handling
- Pierce Renilla-Firefly Luciferase Dual Assay Kit
- Pericolo
- Tossicità acuta Categoria 3
- Liquido infiammabile Categoria 2
- Tossicità specifica per organi bersaglio (esposizione singola) Categoria 1
- H225-Liquido e vapori facilmente infiammabili.
- H301-Tossico se ingerito.
- H311-Tossico per contatto con la pelle.
- H331-Tossico se inalato.
- H370-Provoca danni agli organi.
- P210-Tenere lontano da fonti di calore, superfici riscaldate, scintille, fiamme e altre fonti di innesco. Vietato fumare.
- P241-Utilizzare impianti elettrici/di ventilazione/d'illuminazione/ a prova di esplosione.
- P260-Non respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P301+P310-IN CASO DI INGESTIONE: contattare immediatamente un CENTRO ANTIVELENI/un medico
- P303+P361+P353-IN CASO DI CONTATTO CON LA PELLE (o con i capelli): togliere immediatamente tutti gli indumenti contaminati. Sciacquare la pelle/fare una doccia.
- P304+P340-IN CASO DI INALAZIONE: trasportare l'infortunato all'aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
- P403+P235-Conservare in luogo fresco e ben ventilato.
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
- MIXTURE LIST-Contain: methanol
For Research Use Only. Not for use in diagnostic procedures.
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