Thermo Scientific™ Pierce™ Renilla Luciferase Flash Assay Kit

Features of the Renilla Luciferase Flash Assay Kit:

• Sensitive—greater sensitivity allows utilization of smaller numbers of cells
• Cost effective—highest sensitivity assays result in decreased reagent consumption
• Time-saving—assays require minimal sample handling
• Automation-friendly—amenable to high throughput screens
• Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
• Safe—allows one to perform non-radioactive assays

This Flash Assay Kit contains reagents for measuring the activity of Renilla luciferase in mammalian cell lysates. When used with Thermo Scientific Green Renilla Luc Plasmids, the kit provides an extremely sensitive bioluminescent reporter assay system for intracellular detection of promoter or pathway activity. The signal produced by Green Renilla Luc is considerably greater than signal from either native firefly or Renilla luciferases assayed under similar conditions. Light output captured using a luminometer can be correlated with the amount of Renilla luciferase protein produced and used to determine the activity of the promoter driving Renilla expression.

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as the Varioskan ALF or the Varioskan LUX microplate readers.

Applications:

• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly. The Pierce Renilla Flash Assay Kit reagent optimizes the this signal for use in flash-type luciferase reporter assays. The kit also is compatible for flash assays with other Renilla luciferases and derivatives that use coelenterazine as a substrate.

Related Products

Pierce™ Renilla-Firefly Luciferase Dual Assay Kit (Cat. No. 16185)