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  6. Thermo Scientific™ Pierce™ Renilla Luciferase Glow Assay Kit

Thermo Scientific™ Pierce™ Renilla Luciferase Glow Assay Kit

Codice prodotto. 12172240 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
100 Reaction Kit
1000 Reaction Kit
Dimensione della confezione:
Pezzo
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12172240 100 Reaction Kit Pezzo
12182240 1000 Reaction Kit Pezzo
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Codice prodotto. 12172240 Fornitore Thermo Scientific™ N. del fornitore 16166

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Description

Obtain bright and stable bioluminescence signal (half-life approx. 3 hour) in the presence of Renilla luciferase reporters.

Thermo Scientific™ Pierce Renilla Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of Green Renilla luciferase reporter.

This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of Renilla luciferase in cell lysates. In the presence of luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Green Renilla Luc Plasmids; however, this glow kit may be used to detect the activity of other Renilla luciferase derivatives that use coelenterazine as a substrate.

Highlights

  • Sensitive—highly sensitive detection of Green Renilla luciferase activity
  • Stable—increased signal stability compared to flash assays
  • Simple—does not require a luminometer with injectors
  • Compatible—assay reagents compatible with other Renilla luciferases
  • Automation-friendly—amenable to high throughput assays
  • Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
  • Safe—allows one to perform non-radioactive assays

Requires

Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as the Varioskan ALF or the Varioskan LUX microplate readers

Recommended applications

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

TRUSTED_SUSTAINABILITY

Specifications

Tipo Assay Kit
Bersaglio Luciferase, Green Renilla Luciferase
Quantità 100 Reaction Kit
Content And Storage Upon receipt store kit at -80°C or store individual components as indicated above.
• Renilla Glow Assay Buffer, 5 mL, store at 4°C
• Coelenterazine (100X), 50 μL, store at -80°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature
Saggio Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells Mammalian Cells
Metodo di rilevamento Bioluminescence
Da utilizzare con (apparecchiatura) Luminometer (Microplate)
Formato 384-well plate, 96-well plate
Tipo di etichetta Enzyme Labeled
Substrate Properties Chemical Substrate
Tecnica Enhanced Chemiluminescence
Tipo di substrato Luciferase Substrate
Linea di prodotti Pierce
Substrato Coelenterazine
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Safety and Handling

compliance-icons compliance-icons compliance-icons
Product Identifier
  • Pierce Renilla Luciferase Glow Assay Kit
 Signal Word
  • Pericolo
 Hazard Category
  • Tossicità acuta Categoria 3
  • Liquido infiammabile Categoria 2
  • Tossicità specifica per organi bersaglio (esposizione singola) Categoria 1
 Hazard Statement
  • H225-Liquido e vapori facilmente infiammabili.
  • H301-Tossico se ingerito.
  • H311-Tossico per contatto con la pelle.
  • H331-Tossico se inalato.
  • H370-Provoca danni agli organi.
 Precautionary Statement
  • P210-Tenere lontano da fonti di calore, superfici riscaldate, scintille, fiamme e altre fonti di innesco. Vietato fumare.
  • P241-Utilizzare impianti elettrici/di ventilazione/d'illuminazione/ a prova di esplosione.
  • P260-Non respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
  • P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
  • P301+P310-IN CASO DI INGESTIONE: contattare immediatamente un CENTRO ANTIVELENI/un medico
  • P304+P340-IN CASO DI INALAZIONE: trasportare l'infortunato all'aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
  • P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
  • P405-Conservare sotto chiave.
  • P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
 Supplemental information
  • MIXTURE LIST-Contain: methanol

For Research Use Only. Not for use in diagnostic procedures.

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