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Thermo Scientific™ Kit di estrazione DNA da lievito Pierce™

Quickly, efficiently and easily lyse yeast cells to release DNA with Y-PER lysis reagent.

Marca:  Thermo Scientific™ 78870

158.12 EUR valido fino al 2024-03-30
Usa il codice promo "21648" per avere il tuo prezzo promozionale.



Dettagli aggiuntivi : Peso : 5.44310kg


Codice prodotto. 10473985

  • 236.00€ / 1 set
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Descrizione

Descrizione

Quickly and easily lyse yeast cells to release DNA for study with Thermo Scientific™ Pierce Yeast DNA Extraction Kit, which utilizes a Thermo Scientific γ-PER Reagent.

The kit provides greater yields than traditional methods of DNA isolation from yeast. Typically, extraction and purification of DNA from yeast are time-consuming and labor-intensive. The yeast proteinaceous cell wall is notoriously difficult to lyse and requires harsh treatments that are time-consuming and can damage the extracted DNA. The Yeast DNA Extraction Kit protocol requires less than one hour to complete, is effective without enzymatic treatment or glass beads, and yields little to no RNA contamination regardless of RNase presence. In studies with Saccharomyces cerevisiae, high yields of genomic and plasmid DNA are consistently obtained. DNA purified using this kit is suitable for PCR amplification, bacterial transformations, restriction digestions and hybridization applications.

Highlights:

  • Eliminates need for glass beads or harsh enzyme treatments
  • Yields DNA that is compatible with polymerase chain reaction (PCR) amplification
  • Rapidly isolates plasmid DNA from S. cerevisiae suitable for transformation of E. coli
  • Protocol is easily scalable for use with single colonies or 500mL cultures

Includes:

Thermo Scientific γ-PER Yeast Protein Extraction Reagent, DNA releasing reagents A and B, and protein removal reagent

Specifica

Specifica

Sufficient For: 50 purifications from 10 mL cultures
• Y-PER Yeast Protein Extraction Reagent, 25 mL
• DNA Releasing Agent A, 20 mL
• DNA Releasing Agent B, 20 mL
• Protein Removal Reagent, 10 mL

Store at 4°C. Precipitates may form; gently warm at 37°C for 1–5 minutes.
PCR, Sequencing, Transformation
Yeast DNA Extraction Kit
Genomic DNA
Yeast
Not High-throughput Compatible
75 mL
Suggerimenti di prodotto

Suggerimenti di prodotto

Videos
Sicurezza e movimentazione

Sicurezza e movimentazione

Identificativo prodotto
  • Yeast DNA Extraction Kit

Avvertenza
  • Attenzione

Categoria di pericolo
  • Lesione oculare grave/irritazione oculare Categoria 2

Indicazioni di pericolo
  • H319-Provoca grave irritazione oculare.

Consigli di prudenza
  • P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
  • P264-Lavare accuratamente dopo l'uso.
  • P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.

Informazioni supplementari
  • MIXTURE LIST-Contiene: lithium chloride, sodium dodecyl, trometamol, sodium chloride, sodium chloride,disodium hydrogenort

SDS
Documenti

Documenti

Certificati
Offerte speciali

Offerte speciali

For Research Use Only. Not for use in diagnostic procedures.