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Description
PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.
Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.
Features of PNPP (p-nitrophenyl phosphate):
- One-component alkaline phosphatase (AP) substrate
- Absorbance maximum of 405 nm
- Develops a yellow hue in solution upon reaction with AP
- Safer to use—contains a non-mercury, non-azide preservative
The substrate is offered in several formats:
PNPP Substrate
- Powder Form
PNPP Substrate Tablets
- 5 mg tablets with an inert filler as the balance
PNPP Substrate Kit
- PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.
1-Step™ PNPP Substrate Solution
- Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.
Specifications
Specifications
| Descrizione | PNPP Tablets |
| Product Type | ELISA Substrate |
| Quantità | 105 Tablets |
| Metodo di rilevamento | Colorimetric |
| Forma | Tablet |
| Formato | Tablet |
| Substrate Properties | Chemical Substrate |
| Tipo di substrato | Alkaline Phosphatase |
| Content And Storage | Upon receipt store product at -20°C. |
| Shipping Condition | Wet Ice |
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