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Macherey-Nagel™ Protino Ni-NTA Agarose for His-tag Protein Purification
Descrizione
- Enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
- Proteins from any expression system can be purified under native or denaturing conditions.
- Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
- Consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC™ columns.
- The resin is precharged with Ni2+ ions and therefore ready to use.
- Technology: Immobilized metal ion affinity chromatography
- Batch binding, gravity flow column chromatography, MPLC / FPLC applications
- Matrix: 6 % beaded agarose (cross-linked), precharged with Ni2+
- Bead size: 45 to 165 μm
Specifica
Specifica
| Capacità di legame | <50 mg/mL settled agarose |
| Descrizione | Aqueous suspension |
| Content And Storage | 4°C to 8°C |
| Quantità | 25 mL |
| Da utilizzare con (applicazione) | Purification of His-tag proteins |
| Bersaglio | Protein |
| Tipo | Agarose |
| Tipo di legante | NTA (nitrilotriacetic acid) |
Sicurezza e movimentazione
Avvertenza
- Attenzione
- Liquido infiammabile Categoria 3
- Sensibilizzazione della pelle Categoria 1
- H226-Liquido e vapori infiammabili.
- H317-Può provocare una reazione allergica cutanea.
- P210-Tenere lontano da fonti di calore, superfici riscaldate, scintille, fiamme e altre fonti di innesco. Vietato fumare.
- P261-Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- MIXTURE LIST-Contain: ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU), resin suspension with Ni in
Titolo del prodotto
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