Invitrogen™ Rabbit anti-Human IgG Fc Secondary Antibody, FITC

Rabbit Polyclonal Secondary Antibody

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. This antibody has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Antibody Specificity: This antibody reacts with the heavy chains of human IgG, but not with the light chains on most human immunoglobulins. No antibody was detected against human IgM, IgA, or non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Fluorophore: Fluorescein-5-isothiocyanate (FITC-isomer 1) Amax = 492 nm; Emax = 520 nm Fluorophore/Protein: 7.5 μg/mg; 2.8 moles FITC per mole IgG Restoration and Storage: Store product at 4°C until opened. Restore with 1.1 mL distilled water (1.5 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Country of Origin: USA.