Invitrogen™ Rabbit anti-Sheep IgG (H+L) Secondary Antibody

Rabbit Polyclonal Secondary Antibody

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31240 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31240 reacts with the heavy chains of sheep IgG and with the light chains common to most sheep immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. NOTE: For blocking, we recommend using a 4-5 % normal serum from the same host species of the labeled secondary antibody. The use of BSA and/or dry milk to block or dilute anti-bovine IgG, anti-goat IgG, anti-horse IgG, anti-sheep IgG and/or primary antibodies, may significantly increase background and/or reduce secondary antibody titer since BSA and dry milk may contain IgG which reacts with these antibodies. Country of Origin: USA.