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Rat spleen tissue lysate, Non-denatured; Abnova

Codice prodotto. 16099605
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Quantità:
200 μg
Dimensione della confezione:
200µg
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Codice prodotto. Quantità unitSize
16099605 200 μg 200µg
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Codice prodotto. 16099605 Fornitore Abnova N. del fornitore L042W2.200ug

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Description

Non denatured tissue lysate of Rat spleen

  • Tissue: Spleen
  • Host: Rat
  • Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Spécification

Da utilizzare con (applicazione) Immunoprecipitation, Western Blot
Tissue Spleen
Descrizione Whole tissue lysate (non-denatured)
Tampone di lisi Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Metodo di preparazione Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Analisi di controllo qualità 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantità 200 μg
Tampone di conservazione In modified RIPA Lysis Buffer.
Specie ospite Rat
Concentrazione 5 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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