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Invitrogen™ Rat TNF alpha ELISA Kits
Descrizione
Assay TNFα (rat tumor necrosis factor alpha) levels in rat serum, plasma (EDTA, heparin) and culture supernatant samples with our Thermo Scientific Rat TNF alpha ELISA Kits.
Target: Natural and recombinant rat TNFα (rat tumor necrosis factor alpha)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 31 to 2500pg/mL
Sensitivity: < 15pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)
Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
Specifica
Specifica
| Intervallo saggio | 31 to 2500pg/mL |
| Assay Sensitivity | <15pg/mL |
| Descrizione | Rat TNF alpha ELISA Kit |
| Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
| Tipo di campione | Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum |
| Specificità | No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%) |
| Sufficiente per | 96 microplate wells |
| Contenuto kit | Standard Diluent, 25mL, TMB Substrate Solution, 13mL, Stop Solution, 13mL, Pre-Treatment Buffer, 6mL, Lyophilized Recombinant Rat TNF alpha Standard, 2 vials, Biotinylated Antibody Reagent, 6mL, Streptavidin-HRP Reagent, 14mL, Anti-Rat TNF alpha Precoated 96-well Strip Plate, 1 ea, 30X Wash Buffer, 50mL |
| Misurazione | Absorbance at 450nm minus absorbance at 550nm |
| Precisione | Intra-assay CV <10%; inter-assay CV <10% |
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