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R&D Systems™ Recombinant Human TNF-alpha Protein

Codice prodotto. 18157970
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Quantità:
20 μg
Dimensione della confezione:
20µg
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Codice prodotto. Quantità unitSize
18157970 20 μg 20µg
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Codice prodotto. 18157970 Fornitore R&D Systems™ N. del fornitore 210TA020/CF

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Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL. The specific activity of Recombinant Human TNF-alpha is approximately 7.6 x 104 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786). Specific activity is for reference purposes only and is not routinely tested.
TRUSTED_SUSTAINABILITY

Specifica

Formulazione Lyophilized from a 0.2μm filtered solution in PBS.
ID gene (immissione) 7124
Peso molecolare 17.5 kDa
Nome TNF-alpha
Quantità 20 μg
Sorgente E. coli-derived human TNF-alpha protein Val77-Leu233, with and without an N-terminal Met
Requisiti di stoccaggio Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
Concentrazione di endotossine Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
Alias gene APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Coniugato Unconjugated
Recombinant Recombinant
Purity or Quality Grade >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
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