Thermo Scientific™ Inibitore di RNasi RiboLock

• One unit of the RiboLock RNase Inhibitor inhibits the activity of 5ng RNase A by 50%
• Inhibitor activity is assayed in the following mixture: 100mM Tris-HCl (pH 7.5), 1.2mM EDTA, 0.1mg/mL BSA, 100ng/mL RNase A, 0.1mg/mL [³H]-RNA, 50mg/mL yeast RNA, 8mM DTT

• The enzyme is supplied in: 20mM HEPES-NaOH (pH 7.5), 50mM NaCl, 8mM DTT, 0.5mM ELUGENT Detergent and 50% (v/v) glycerol

• Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound
• Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min. heating at 70°

Recommended for:

• Inhibition of RNA degradation in the following: in vitrotranscription (1), cDNA synthesis (2), in vitrotranslation (3), isolation of mammalian cell fractions that contain mRNA-protein complex
• RNA purification and storage
• Separation and identification of specific ribonuclease activities
• Studies of tumor suppression

Note:

• DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity
• Recommended concentration 1u/μl of a reaction mixture