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  5. IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector

IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector
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Codice prodotto. 15613888 Sfoglia Tutto IBA Lifesciences Prodotti
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Vettore:
pDSG-IBA102
pDSG-IBA103
pDSG-IBA104
pDSG-IBA105
pDSG-IBA43
pDSG-IBA45
pDSG-IBAwt1
pDSG-IBAwt2
Protein Tag:
His Tag (6x), Strep-tag II
Twin-Strep-tag
Untagged
Dimensione della confezione:
5µg
8 product options available for selection
Product selection table with 8 available options. Use arrow keys to navigate and Enter or Space to select.
Codice prodotto. Vettore Protein Tag unitSize
15613888 pDSG-IBA102 Twin-Strep-tag 5µg
15603888 pDSG-IBA45 His Tag (6x), Strep-tag II 5µg
15623888 pDSG-IBA103 Twin-Strep-tag 5µg
15633888 pDSG-IBA104 Twin-Strep-tag 5µg
15643888 pDSG-IBA105 Twin-Strep-tag 5µg
15663878 pDSG-IBAwt1 Untagged 5µg
15673878 pDSG-IBAwt2 Untagged 5µg
15693878 pDSG-IBA43 His Tag (6x), Strep-tag II 5µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 8 options available.
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Codice prodotto. 15613888 Fornitore IBA Lifesciences N. del fornitore 55219001

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Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso

Descrizione

Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA Lifesciences GmbH Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.

pDSG-IBA vectors for MEXi mammalian expression system

  • Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
  • Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
    • Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number

Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Tekniske data

Sistema costitutivo o inducibile Constitutive
Gene marcatore Ampicillin
Promotore CMV
Product Type Vector
Agente di selezione (eucariotico) Geneticin™ (G-418)
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Specie Mammalian
Protein Tag Twin-Strep-tag
Cloning Method Direct Cloning
Concentrazione 250 ng/μL
Primer Forward
Quantità 5 μg
Vettore pDSG-IBA102
Da utilizzare con (applicazione) BM40 secretion signal
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