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  5. IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector

IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector
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Codice prodotto. 15673878 Sfoglia Tutto IBA Lifesciences Prodotti
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Vettore:
pDSG-IBA102
pDSG-IBA103
pDSG-IBA104
pDSG-IBA105
pDSG-IBA43
pDSG-IBA45
pDSG-IBAwt1
pDSG-IBAwt2
Protein Tag:
His Tag (6x), Strep-tag II
Twin-Strep-tag
Untagged
Unit Size:
5µg
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Product Code. 15673878

Brand: IBA Lifesciences 55201001

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Description

Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA Lifesciences GmbH Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.

pDSG-IBA vectors for MEXi mammalian expression system

  • Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
  • Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
    • Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number

Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

Sistema costitutivo o inducibile Constitutive
Gene marcatore Ampicillin
Promotore CMV
Product Type Vector
Agente di selezione (eucariotico) Geneticin™ (G-418)
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Specie Mammalian
Protein Tag Untagged
Cloning Method Direct Cloning
Concentrazione 250 ng/μL
Primer pENTRy-IBA51 Reverse Sequencing
Quantità 5 μg
Vettore pDSG-IBAwt2
Da utilizzare con (applicazione) BM40 secretion signal
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