Sodium Phosphate Monobasic, Monohydrate, MP Biomedicals™

Sodium phosphate occurs in several forms: monobasic (NaH2PO4), dibasic (Na2HPO4), and tribasic (Na3PO4). Most neutral sodium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.

• A table for preparation of 0.1M sodium phosphate buffer at 25°C using various proportions of sodium phosphate monobasic and sodium phosphate dibasic has been published.
• A study of the effect of freeze-thaw storage cycles on proteins in sodium phosphate and potassium phosphate buffer solutions has been reported.
• The effect of 5mM sodium phosphate on the efficacy of electrospray ionization (ESI) ion mobility spectrometry (IMS) analysis has been evaluated.
• A protocol for the purification of pyrogen-free mouse IgG1 monoclonal antibodies which uses 10 mM sodium phosphate (pH 7.4) has been published.
• An ion-pairing HPLC method for the analysis of 5-aminosalicylic acid has been reported.
• A TLC method for separation of nucleotide sugars in the study of glycosyltransferase activity has been published.

Sodium phosphate monobasic, monohydrate has a wide variety of uses:

• Sequestrant, emulsifier and buffer in foods
• Mordant in dyeing
• Weighting silk in tanning
• Manufacture of enamels, ceramics, detergents, boiler compounds
• Fireproofing agent
• In soldering and brazing instead of borax
• Reagent and buffer in analytical chemistry
• Cathartic
• Laxative