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Sol8 (mouse myoblast) Whole cell lysate, Non-denatured, Abnova

Codice prodotto. 16079635
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Quantità:
200 μg
Dimensione della confezione:
200µg
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Codice prodotto. Quantità unitSize
16079635 200 μg 200µg
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Codice prodotto. 16079635 Fornitore Abnova N. del fornitore L058V2.200ug

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Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso

Descrizione

Non-denatured whole cell lysate of Sol8 (mouse myoblast)

  • Tissue: Skeletal Muscle
  • Host: Mouse
  • Lysis Buffer: Modified RIPA Lysis Buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In modified RIPA Lysis Buffer

Western Blotting, Immunoprecipitation

Specifica

Da utilizzare con (applicazione) Immunoprecipitation, Western Blot
Tissue Skeletal Muscle
Descrizione Whole cell lysate (non-denatured)
Tampone di lisi Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Metodo di preparazione Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Analisi di controllo qualità 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantità 200 μg
Tampone di conservazione In modified RIPA Lysis Buffer.
Specie ospite Mouse
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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