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Thermo Scientific™ Spectra™ Multicolor High Range Protein Ladder

Codice prodotto. 11872124
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Quantità:
2 x 250 μL
Dimensione della confezione:
pezzi 2
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Codice prodotto. Quantità unitSize
11872124 2 x 250 μL pezzi 2
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Codice prodotto. 11872124 Fornitore Thermo Scientific™ N. del fornitore 26625

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Descrizione

A mixture of eight blue-, green- and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.

Thermo Scientific™ Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Three different chromophores (blue, orange, green) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required.

Highlights:

  • Size range – eight proteins spanning 40 to 300kDa

  • Multicolor – three different colors for unambiguous band-size assignment

  • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

  • Sharp bands – color-coded bands of similar intensity for easy visualization

  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

  • Membrane-compatible – colored bands transfer to membranes for Western blotting

Includes:

Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.

Recommended for:

Monitoring of protein migration during SDS-PAGE; Verifying Western transfer efficiency; Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots; Locating a protein of interest for excision from an unstained preparative gel

TRUSTED_SUSTAINABILITY

Specifica

Content And Storage Contents: two vials of 250 μL each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C
Metodo di rilevamento Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
Number of Markers 8
Ready to Load Yes
Recommended Applications Tris-acetate gels
Intervallo dimensioni 40 to 300 kDa
Da utilizzare con (applicazione) Tris-acetate gels
Compatibilità gel Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Peso molecolare 300, 250, 180, 130, 100, 70, 50, 40 kDa
Quantità 2 x 250 μL
Shipping Condition Approved for shipment on Wet or Dry Ice
Product Line Spectra
Product Type Protein Ladder
Stain Type Blue, Orange, Green
System Type Western Blotting, SDS-PAGE
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For Research Use Only. Not for use in diagnostic procedures.

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