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IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo
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Vettore:
pLSG-IBA 102
pLSG-IBA 103
pLSG-IBA 104
pLSG-IBA 105
pLSG-IBA 123
pLSG-IBA 142
pLSG-IBA 143
pLSG-IBA 144
pLSG-IBA 145
pLSG-IBA 162
pLSG-IBA 164
pLSG-IBA 167
pLSG-IBA 168
pLSG-IBA 23
pLSG-IBA 25
pLSG-IBA 3
pLSG-IBA 33
pLSG-IBA 35
pLSG-IBA 43
pLSG-IBA 45
pLSG-IBA 5
pLSG-IBA 62
pLSG-IBA 63
pLSG-IBA 64
pLSG-IBA 65
pLSG-IBA wt1
pLSG-IBA wt2
Dimensione della confezione:
5µg
Descrizione
pLSG-IBA Baculo virus (insect cells) expression vectors
The pLSG-IBAwt1 vector carries the Polyhedrin promoter for high-level expression in insect cells.
- Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild type virus to great extent.
- Cloning and expression of recombinant proteins in insect cells
- Polyhedrin promoter drives high-level expression of the insert
- Ampicillin resistance and ColE1 origin of replication (pUC) support propagation in E. coli
Please note that cloning into pLSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pLSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Specifica
Specifica
| Formato | Liquid |
| Da utilizzare con (apparecchiatura) | StarGate™ System, Insect Host Cells |
| Agente inducente | BacPAK6 linearized A cmNPV DNA (Clontech) or circular flashBAC modified A cmNPV DNA (Oxford Expression Technologies) |
| Include | pUC ori, Ampicillin resistance |
| pH | 8 |
| Promotore | Polyhedrin |
| Product Type | Acceptor Vector for bacula (insect cell) expression |
| Tampone di conservazione | 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg |
| Content And Storage | 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Protein Tag | Untagged |
| Vedi altri risultati |
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