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  5. Thermo Scientific™ Substrato chemiluminescente SuperSignal™ West Dura

Thermo Scientific™ Substrato chemiluminescente SuperSignal™ West Dura

Codice prodotto. 10445345 Sfoglia Tutto Thermo Scientific Prodotti
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Codice prodotto. 10445345 Fornitore Thermo Scientific™ N. del fornitore 34075

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300.73 EUR valido fino al 2026-05-08
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Description

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Thermo Scientific™ SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems and quantitation. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, enabling multiple film or camera exposures.

SuperSignal West Dura Extended Duration Substrate characteristics include:

  • Sensitivity: mid-femtogram
  • Stability: 24-hr working solution stability; 1-year kit stability at room temperature
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: 24 hours
  • Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
  • Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)
TRUSTED_SUSTAINABILITY

Specifications

Product Type Substrate
Metodo di rilevamento Chemiluminescence
Forma Liquid
Da utilizzare con (applicazione) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line SuperSignal
Quantità Kit da 100 ml
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1μg/mL)
Sensibilità Mid-femtogram
Signal Duration 24 hr.
Sufficiente per 12 Mini-Gel Blots, 1000 sq. cm of Membrane
Contenuto kit Tampone di perossido stabile, 50 ml, Stable Peroxide Buffer, 50mL
Content And Storage Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

Store at room temperature.
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For Research Use Only. Not for use in diagnostic procedures.

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