Thermo Scientific™ Substrato chemiluminescente SuperSignal™ West Femto

Highlights:

• ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

• Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes

• Quantitative – produces quantitative signal that is measurable over two orders of magnitude

• Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)

• Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system

• Excellent signal duration – 8 hours of usable light-output when conditions are optimized

• Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature