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SW-13 (human small cell carcinoma of the adrenal cortex) Whole cell lysate, Denatured; Abnova

Codice prodotto. 16019505
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Quantità:
200 μg
Dimensione della confezione:
200µg
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Codice prodotto. Quantità unitSize
16019505 200 μg 200µg
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Codice prodotto. 16019505 Fornitore Abnova N. del fornitore L005V1.200ug

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Descrizione

Western Blotting

Specifica

Da utilizzare con (applicazione) Western Blot
Tissue Adrenal Gland (Cortex)
Descrizione Whole cell lysate (denatured)
Tampone di lisi Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Metodo di preparazione Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Analisi di controllo qualità 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantità 200 μg
Tampone di conservazione In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Specie ospite Human
Concentrazione 3 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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